东北铁线莲中抗肿瘤活性成分的提取工艺优选

金晨; 翟兴英; 李翔; 张武岗; 唐琍萍; 张凌; 冯育林; 杨世林

doi:10.11653/syfj2013080033

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东北铁线莲中抗肿瘤活性成分的提取工艺优选

更新时间：2024-01-04

- 东北铁线莲中抗肿瘤活性成分的提取工艺优选
- Optimization of Extraction Process of Anti-tumor Active Ingredient in Rupr
- 中国实验方剂学杂志 2013年19卷第8期页码：33-36
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- DOI：10.11653/syfj2013080033
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- 纸质出版日期：2013
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金晨, 翟兴英, 李翔, 等. 东北铁线莲中抗肿瘤活性成分的提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(8):33-36.

JIN Chen, ZHAI Xing-ying, LI Xiang, et al. Optimization of Extraction Process of Anti-tumor Active Ingredient in Rupr[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 33-36.
金晨, 翟兴英, 李翔, 等. 东北铁线莲中抗肿瘤活性成分的提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(8):33-36. DOI： 10.11653/syfj2013080033.

JIN Chen, ZHAI Xing-ying, LI Xiang, et al. Optimization of Extraction Process of Anti-tumor Active Ingredient in Rupr[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 33-36. DOI： 10.11653/syfj2013080033.

摘要

目的: 建立东北铁线莲中抗肿瘤活性成分W2[3-O-α-L-吡喃鼠李糖-(1→6)-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃葡萄糖-(1→4)-β-D-吡喃核糖-(1→3)-α-L-吡喃鼠李糖-(1→2)-α-L-吡喃阿拉伯糖齐墩果酸皂苷]的提取工艺。方法: 以W2提取量为指标

采用HPLC测定W2含量

通过正交试验和单因素试验考察提取溶媒、提取时间、料液比、提取次数对提取工艺的影响

确定W2最佳提取工艺。结果: 最佳提取工艺为30%乙醇提取2.5 h

料液比1:12

提取2次。结论: 优选的提取工艺合理、稳定、可行

为东北铁线莲后续研究提供实验依据。

Abstract

Objective: To establish extraction technology for anti-tumor active ingredient of W2 [3-O-α-L-pyran rhamnose-(1→6)-β-D-glucopyranosyl-(1→4)-β-D-glucopyranosyl-(1→4)-β-D-ribopyranose-(1→3)-α-L-pyran rhamnose-(1→2)-α-L-arabinopyranosyl oleanolic acid saponins] from Clematis manshurica Rupr. Method: With the content of W2 as index

which was determined by HPLC

effects of extraction time

extraction solvent

liquid-solid ratio and extraction times on extraction technology were investigated by single factor test and orthogonal test

then to determine optimum technology of W2.Result: Optimum extraction process of W2 was as following:reflux extracted 2 times with 12 times the amount of 30% ethanol

2.5 hours per time.Conclusion: Optimized technology was reasonable

stable and feasible

which could provide a experimental basis for the follow-up research of C. manshurica Rupr.

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