Objective: To optimize water extraction and alcohol precipitation technology of Kubai Fuyan suppositories. Method: Water extraction and alcohol precipitation method was adopted

with the quality of matrine

berberine and osthole as indexes

effects of liquid-solid ratio

extraction times and time on water extraction technology was investigated by orthogonal test;With composite score of the quality of matrine

berberine and osthole as index

the relatively density of concentrated liquid

the concentration of alcohol precipitation and storage time were chosen as factors

alcohol precipitation technology was optimized by orthogonal test. Result: Optimum extraction process was as following:extracted 3 times with 12 times the amount of water

2 hours each time;Optimized alcohol precipitation technology was:the relative density of concentrated liquid 1.18 (25 ℃)

the concentration of alcohol precipitation 60%

placed 12 hours. Conclusion: These optimized technologies was stable

reasonable and feasible

them could provide experimental basis for clinical application of Kubai Fuyan suppositories.