芪苈强心胶囊UPLC指纹图谱研究

姜新刚; 贾继明; 李叶双; 乔莉

doi:10.11653/syfj2013080080

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芪苈强心胶囊UPLC指纹图谱研究

更新时间：2024-01-04

- 芪苈强心胶囊UPLC指纹图谱研究
- UPLC Fingerprints of Qili Qiangxin Capsule
- 中国实验方剂学杂志 2013年19卷第8期页码：80-83
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家重点基础研究发展计划(973计划)项目(2012CB518606);国家十二五"重大新药创制"科技重大专项(2011ZX09401-020);国家自然科学基金青年基金项目(81102768)
- DOI：10.11653/syfj2013080080
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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姜新刚, 贾继明, 李叶双, 等. 芪苈强心胶囊UPLC指纹图谱研究[J]. 中国实验方剂学杂志, 2013,19(8):80-83.

JIANG Xin-gang, JIA Ji-ming, LI Ye-shuang, et al. UPLC Fingerprints of Qili Qiangxin Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 80-83.
姜新刚, 贾继明, 李叶双, 等. 芪苈强心胶囊UPLC指纹图谱研究[J]. 中国实验方剂学杂志, 2013,19(8):80-83. DOI： 10.11653/syfj2013080080.

JIANG Xin-gang, JIA Ji-ming, LI Ye-shuang, et al. UPLC Fingerprints of Qili Qiangxin Capsule[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 80-83. DOI： 10.11653/syfj2013080080.

摘要

目的: 采用超高效液相色谱(UPLC)法建立芪苈强心胶囊的指纹图谱。方法: 采用Waters ACQUITY UPLC BEH C18色谱柱(2.1 mm×100 mm

1.7 μm)

流动相乙腈-0.15%磷酸水

梯度洗脱

流速0.5 mL·min-1

柱温40 ℃

检测波长203 nm。结果: 建立了芪苈强心胶囊的UPLC指纹图谱

共标定了36个共有峰

指认了其中8个共有峰

10批芪苈强心胶囊指纹图谱的相似度均在0.979～1.000。结论: 该法具有良好的精密度、稳定性和重复性

为提高芪苈强心胶囊质量控制提供了新方法。

Abstract

Objective: To develop a ultra performance liquid chromatography (UP-LC) method for fingerprints of Qili Qiangxin Capsule. Method: The Waters Acquity UPLC BEH C18 (2.1 mm×100 mm

1.7 μm) column was used. Acetonitrile and water containing 0.15% phosphoric acid were used as mobile phase of UPLC with gradient elution. The detection wavelength was set at 203 nm. Flow rate was set at 0.5 mL·min-1 and column temperature was maintained at 40 ℃. Result: UPLC fingerprint was established with 36 common peaks

8 of 36 common peaks were identified

and the similar degrees of the UPLC fingerprints of Qili Qiangxin capsule from 10 batches were between 0.979 and 1.000. Conclusion: The method of fingerprints has acceptable precision

reproducibility and stability

which can be used as a new means for the quality control of Qili Qiangxin capsule.

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