不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响

邱新建; 赵海鹏; 刘向东; 苏瑞强; 李守信; 赵志全

doi:10.11653/syfj2013080083

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不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响

更新时间：2024-01-04

- 不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响
- Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive
- 中国实验方剂学杂志 2013年19卷第8期页码：83-86
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- 基金信息：
  
  国家重点基础研究发展计划(973计划)项目(2012CB724001)
- DOI：10.11653/syfj2013080083
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- 纸质出版日期：2013
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邱新建, 赵海鹏, 刘向东, 等. 不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响[J]. 中国实验方剂学杂志, 2013,19(8):83-86.

QIU Xin-jian, ZHAO Hai-peng, LIU Xiang-dong, et al. Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 83-86.
邱新建, 赵海鹏, 刘向东, 等. 不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分含量的影响[J]. 中国实验方剂学杂志, 2013,19(8):83-86. DOI： 10.11653/syfj2013080083.

QIU Xin-jian, ZHAO Hai-peng, LIU Xiang-dong, et al. Effects of Different Purification Methods on Content of Caffeoyl Quinic Acids from Flos Lonicerae Extractive[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(8): 83-86. DOI： 10.11653/syfj2013080083.

摘要

目的: 考察不同纯化方法对金银花提取物中咖啡酰奎宁酸类成分的影响。方法: 应用醇沉法、石硫法、石硫醇法、大孔树脂吸附法及萃取法等对金银花进行提取纯化

并采用高效液相色谱法测定其中6种咖啡酰奎宁酸类成分的含量。结果: 萃取法所得金银花提取物中咖啡酰奎宁酸类成分的含量最高

总咖啡酰奎宁酸含量达83.91%。结论: 萃取法是制备金银花提取物的理想方法。

Abstract

Objective: To study the effects of different purification ways on the extractive rate of caffeoyl quinic acids in Flos Lonicerae extractive. Method: Several purification processes were used respectively in the preparation of Flos Lonicerae extractive

such as alcohol precipitation method

lime-sulfur method

lime-sulfur ethanol method

macroporous resin method

and extraction method etc. The content of the six caffeoyl quinic acids was determined by HPLC. Result: The content of the caffeoyl quinic acids in Flos Lonicerae extractive by extraction method was higher than the other methods. Total content of caffeoyl quinic acids was up to 83.91%. Conclusion: The extraction method is the best in the preparation of Flos Lonicerae extractive.

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