Objective: A reversed-phase high performance liquid chromatographic method (RP-HPLC) was developed for the simultaneous analysis of six isoflavonoids of Gegen

the roots of Puerariae lobatae. Method: HPLC determination was performed on a Kromasil C18 column (4.6 mm×250 mm

5 μm) and detected at 250 nm. The mobile phase was consisted of methanol and 0.1% citric acid solution with gradient elution.The flow rate was 1.0 mL·min-1

and column temperature was kept at 30 ℃. Result: The method was proved to be linear in the ranges of 76.2-1 016

65.8-1 316

85.6-1 712

49.98-499.8

21.94-219.4

48.5-485 ng for the six isoflavonoid

3'-hydroxypuerarin

puerarin

daidzin

genistin

ononin

daidzein

respectively. The average recoveries were 99.73% (RSD 0.6%)

100.48%(RSD 1.11%)

100.37%(RSD 0.79%)

100.28%(RSD 1.07%)

99.96%(RSD 1.22%)

97.36%(RSD 1.59%)

respectively. Conclusion: Results obtained showed that the method is convenient

accurate and reliable

can be applied to the simultaneous determination of the six isoflavonoids in Radix Puerariae lobatae.