Objective: This project aimed to find if prescription for tonifying kidney fed during embryonic development can prevent impaired glucose tolerance(IGT) caused by feeding high fat diet and its influence on insulin and leptin. Method: The pregnant rats were randomly divided into 5 groups. The normal control group and model group were fed by normal saline on the 1st day

ig

20 mL·kg-1.In other 3 experiment groups

the pregnant rats were fed respectively with Zuogui Wan (ZGW

a prescription for reinforcing kidney Yin) or Yougui Wan(YGW

a prescription for reinforcing kidney Yang)or Bazhen decoction (BZD

a prescription for supplementing Qi and Blood)

ig

20 g·kg-1

20 mL·kg-1

at the same time. After birth

the offsping rats in the other four groups except the control group were fed with high fat diet in 6th week. After 12 weeks of high fat diet

the fasting blood glucose(FBG)

2 h blood glucose(2 hBG)

fasting serum insulin and serum leptin concentrations were measured. Result: 2 hBG of ZGW group was significant lower than 2 hBG of model group(P<0.05);2 hBG of ZGW group and YGW group was not significantly different from model group.HOMA-IR of ZGW group

YGW group and BZD group was significantly lower than HOMA-IR of model group(P<0.01

P<0.01

P<0.05).Leptin of ZGW group and YGW group was significantly lower than leptin of model group (P<0.01

P<0.01). Leptin of BZD group was not significantly different from model group. Conclusion: ZGW and YGW during embryonic development can all withstand the adverse changes of serum insulin

and ZGW can prevent IGT caused by high fat diet

but YGW can not.BZD during embryonic development can also withstand the adverse changes of serum insulin and can not withstand the adverse changes of serum leptin

which is not as good as ZGW and YGW

and can not prevent IGT caused by high fat diet.