Objective: Tocomparie effect of blood deficiency and HPLC fingerprint chromatogram between Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata

and to findout the material foundationof effects. Method: According to random design

rats were divided into 4 groups. Except the normal control group

the other groups were subcutaneously injected acetyl phenyl hydrazine 0.015 g·kg-1 and 0.03 g·kg-1 in the 3rd day and the 6th day. From the 6th day

rats were intraperitoneally injected CTX for 3 day. During the period of modeling

treatment groups received corresponding drugs at dose of 2 g·kg-1. Then

the effect of Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata were observed

and the fingerprint chromatogram of the water extracted from Polygoni Multiflori Radix and Polygoni Multiflori Radix Preparata

respectively

were established by HPLC. Result: Compared with the normal control group

the results showed that the red blood cell count(RBC)

hemoglobin(Hgb)

hernatocrit(HCT) figures in model group rats declined significantly(P<0.05). Compared with the model group

the results showed that Polygoni Multiflori Radix could not increase RBC and Hgb in anemia model rats

but the HCT was increased(P<0.05)

while Polygoni Multiflori Radix Preparata could rise RBC Hgb and HCT significantly in anemia model rats (P<0.01 or P<0.05). The fingerprint chromatograms result revealed that there were differences not only in chemical consistence but in n determination of constituents between two herbals. Conclusion: The results illustrate that Polygoni Multiflori Radix Preparata has significant blood-nourishing ability. Additionally

the result of the fingerprint chromatogram indicates that the product of Maillard reaction and moderate drug properties are both the possible reason why Polygoni Multiflori Radix Preparata has the better efficacy on blood deficiency.