Objective: To optimize extraction process of compound Weishu capsules(CWC) and establish its quality standard. Method: With yield of dry extract

berberine hydrochloride(BH) and palmatine hydrochloride(PH) as comprehensive evaluation index

orthogonal design was carried out to optimize extraction process by taking the concentration of ethanol

extraction time and liquid-solid ratio as factors

extraction times was investigated by single factor test.Coptidis Rhizoma and Euodiae Fructus in CWC were identified by TLC

the content of BH and PH were determined by HPLC. Result: Optimum extraction process was as following:reflux extracted 3 times with 11 times the amount of 60% ethanol with 1 h each time.TLC identification spots were fairly clear and the blank test showed no interference.The linear range of BH and PH was 3.156-202 and 1.375-88 mg·L-1

respectively with r=0.999 9;The average recovery was 100.2% and 99.7%

respectively with RSD of 1.76% and 1.89%. Conclusion: This optimized technology was stable and feasible with good repeatability

and it could be used in industrial production of CWC. This established quality control method was simple

special

reproducible

and effective for quality control of CWC.