Objective: To establish quantitative and qualitative determination for the quality control standard of K-CoxB-JN. Method: TLC was used to identify Panacis Quiaquefolii Radix

Ophiopogonis Radix

Raphani Semen and Vaccariae Semen. The content of ginsenoside Rg1

Re

Rb1 in K-CoxB-JN was determined by HPLC-ELSD. An Agilent ZORBAX XDB C18 (4.6 mm×250 mm

5 μm) was used

and the mobile phase consisted of acetonitrile-0.2% formic acid (gradient elution). The temperature was set at room condition. The parameter of evaporative light-scattering detector: the temperature of drift tube was at 50℃ and the nebulizer nitrogen flow rate was 2.0 L·min-1; gain was 1. Result: The TLC spots were highly clear without the interference of negative sample

and had good separation effect. The linear relationship was obtained within the range of 0.75-3.75

4.5-30 and 6-40 μg for ginsenoside Rg1

Re

Rb1 respectively (r>0.999). The average recoveries of the three components were 97.10% (RSD 3.96%)

95.15% (RSD 2.21%)

95.60% (RSD 3.00%) respectively. Conclusion: The method appeared to be simple

accurate

sensitive and reliable

and can be used in qualitative and quantitative analysis of K-CoxB-JN.