Objective: To evaluate the quality of cultivated Gentiana in Ningxia Province by the comparative study of effective components in different varieties and wild Gentiana. Method: The content of gentiopicroside and loganin acid was determined according to the method listed in 2010 version of Chinese Pharmacopoeia. The amino acid content was determined by automatic amino acid analyzer

and the content of polysaccharide was determined by anthrone-sulfuric acid method. Result: The content of gentiopicroside and loganin acid in three varieties of Gentiana cultivated in Ningxia was higher than prescribed limits in Chinese Pharmacopoeia

and there were no significant differences in amino acid content

and there were significant differences in the content of polysaccharides compared with wild Gentiana. Conclusion: The quality of three kinds of Gentiana macrophylla cultivated in Ningxia is good. The medicinal value of amino acids and polysaccharides in Gentiana macrophylla can be further researched and utilized.