HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量

魏悦; 李晓; 李自红; 范毅; 张海艳; 赵天增

doi:10.11653/syfj2013090108

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HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量

更新时间：2024-09-23

- HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量
- Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC
- 中国实验方剂学杂志 2013年19卷第9期页码：108-111
- 作者机构：
- 作者简介：
- 基金信息：
  
  河南省科技攻关项目(092102310016;112101310600)
- DOI：10.11653/syfj2013090108
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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魏悦, 李晓, 李自红, 等. HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量[J]. 中国实验方剂学杂志, 2013,19(9):108-111.

WEI Yue, LI Xiao, LI Zi-hong, et al. Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 108-111.
魏悦, 李晓, 李自红, 等. HPLC同时测定连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量[J]. 中国实验方剂学杂志, 2013,19(9):108-111. DOI： 10.11653/syfj2013090108.

WEI Yue, LI Xiao, LI Zi-hong, et al. Simultaneous Determination of Rutin, Forsythoside A and Phillyrin of Forsythiae Fructus and its Extract by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(9): 108-111. DOI： 10.11653/syfj2013090108.

摘要

目的: 建立连翘药材及提取物中芦丁、连翘酯苷A和连翘苷的含量测定方法。方法: 应用高效液相色谱-二极管阵列检测法(HPLC-DAD)

根据各类成分紫外吸收光谱的差异

分别在354

329

277 nm 波长下检测芦丁、连翘酯苷A和连翘苷。采用Sunfire C18 (4.6 mm×150 mm

5 μm)色谱柱

流动相为乙腈(A)-0.2%冰醋酸溶液(B)

梯度洗脱(0 min

14%A;15 min

18%A;40 min

30%A)

流速1 mL·min-1

柱温40℃。结果: 芦丁、连翘酯苷A和连翘苷分别在0.098~4.9

0.24~12.0

0.1~5.0 μg线性关系良好

平均回收率分别为98.63%

98.97%

102.18%

RSD分别为7.94%

0.93%

1.63%。结论: 方法精密度高、重复性好

能够应用于连翘药材及提取物的质量控制。

Abstract

Objective: To establish a HPLC method for simultaneous determination of rutin

forsythoside A and phillyrin of Forsythiae Fructus and its extract. Method: The procedure of HPLC-PDA was performed on the chromatographic column of Sunfire C18 (4.6 mm×150 mm

5 μm)

and the mobile phase in the gradient elution (0 min

14%A; 15 min

18%A; 40 min

30%A) was acetonitrile(A)-0.2% acetic acid solution (B). The flow rate was 1.0 mL·min-1; the temperature was kept at 40℃

and the detection wavelength was set at 354

329 nm and 277 nm respectively. Result: Rutin

forsythoside A and phillyrin were linear in the range of 0.1-2.0

0.12-2.4 μg and 0.05-1.0 μg respectively. The average recoveries were 98.63%

98.97% and 102.18% respectively (RSD 7.94%

0.93% and 1.63%). Conclusion: The method is accurate with higher repeatability

which can be used in the quality control of Forsythiae Fructus and its extract.

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