Objective: To identify the effect of rat serum containing different concentrations of Buzhong Yiqi decoction(BZYQT) on reverse the chemotherapeutic resistance in A549/DDP cells and on the expression of mTOR. Method: Animals were randomized to prepared the rat serum containing varied does levels of BZYQT(does for 11.34

5.67

2.83 g·kg-1·d-1)

the A549

A549/DDP were equally divided into six groups in random: the DDP group

BZYQT low does+DDP group

BZYQT middle does+DDP group

BZYQT high does+DDP group

NS+DDP group and the cell control group.Followed by 15%serum of every group and the different concentrations of DDP(10

20

40

80

100

200 μmol·L-1) for 48 h

assayed by MTT to detect the rat serum containing of BZYQT reverse the chemotherapeutic resistance in A549/DDP.The expression of mTOR in cells were evaluated by indirect immunofluorescence

immunocytochemistry technique and Western-blot. Result: The median inhibitory concentration IC50 and resistant multiple of A549/DDP is significant reduction by the serum containing varied does levels of BZYQT(P<0.05)

especially the effect of the middle dose group is the most significant(P<0.01)

the optical density and fluorescence intensity of mTOR were significantly reduced(P<0.01) and the protein expression levels were significantly lower in the cells(P<0.01). Conclusion: BZYQT appeared to increase the chemotherapeutic sensitivity of A549/DDP cells

showing regression of multiple chemotherapeutic resistance in pulmonary adenocarcinoma by down-regulating expression levels of mTOR.