Objective: To investigate the influence of artesunate (ART) on invasion and apoptosis of novel human palatal salivary gland cell line of adenoid cystic carcinoma (NACC). Method: After treated with ART 100 mg·L-1

apoptosis cells were observed after loaded with acridine orange/ethidium bromide dye (AO/EB). The anti-invasion effect of ART at 25

50

100 mg·L-1 were examined by transwell assay. The expression of vascular endothelial growth factor (VEGF) and its mRNA of NACC cells was evaluated by immunohistochemistry and RT-qPCR technique separately. Result: ①Compared with the control group

apoptosis cells were observed after treated with ART 100 mg·L-1

with irregular and shrinked nucleolus; ②The result of transwell assay showed that ART at 25

50

100 mg·L-1 could inhibit the transmembrane cells obviously compared with control group(P<0.01); ③Compared with the control group

the VEGF protein expression decreased obviously after stimulated with ART (P<0.01); ④Same result were observed in VEGF mRNA expression by RT-qPCR (P<0.01). Conclusion: ART could induce apoptosis

decrease VEGF expression and invasion of NACC cells in vitro.