Objective: To synthesize galactose ligand in non-aqueous medium by enzyme-catalyzed method. Method: Structure of the product was confirmed by IR

ESI-MS

1H-NMR and1C-NMR;With conversion rate of substrate as index

effects of enzyme species

reaction medium

the amount of enzyme

molar ratio of substrate

reaction temperature on ligand synthesis was investigated by single factor tests. Result: Characterization by catalytic reaction

C1' bit hydroxyl of galactose occurred esterification

the received products was the target product.Optimized reaction conditions were as followings:tetrahydrofuran(THF)-dimethyl sulfoxide(DMSO)3:1 as reaction medium

Novozym 435 immobilized lipase as catalyst

the amount of enzyme of 600 U·mL-1

molar ratio of galactose to vinyl stearate at 1:4

and reacted at 55 ℃ for 8 h

the conversion rate of galactose reached more than 70%. Conclusion: Enzyme catalysis method could be used for synthesizing ligand of liver targeted liposomes.