响应面法优选刺槐花中刺槐苷提取工艺

初正云; 郑永芹; 程军

doi:10.11653/syfj2013100024

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响应面法优选刺槐花中刺槐苷提取工艺

更新时间：2024-01-04

- 响应面法优选刺槐花中刺槐苷提取工艺
- Optimization of Extraction Technology for Robinin from Flowers of by Response Surface Method
- 中国实验方剂学杂志 2013年19卷第10期页码：24-27
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省科技攻关项目(2009209001)
- DOI：10.11653/syfj2013100024
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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初正云, 郑永芹, 程军. 响应面法优选刺槐花中刺槐苷提取工艺[J]. 中国实验方剂学杂志, 2013,19(10):24-27.

CHU Zheng-yun, ZHENG Yong-qin, CHENG Jun. Optimization of Extraction Technology for Robinin from Flowers of by Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 24-27.
初正云, 郑永芹, 程军. 响应面法优选刺槐花中刺槐苷提取工艺[J]. 中国实验方剂学杂志, 2013,19(10):24-27. DOI： 10.11653/syfj2013100024.

CHU Zheng-yun, ZHENG Yong-qin, CHENG Jun. Optimization of Extraction Technology for Robinin from Flowers of by Response Surface Method[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 24-27. DOI： 10.11653/syfj2013100024.

摘要

目的: 优化刺槐花中刺槐苷的提取工艺。方法: 根据Box-Benhnken中心组合设计原理

采用响应面分析法优化乙醇体积分数、液料比、浸提时间及提取次数4个自变量对刺槐苷得率的影响

利用SAS 9.2软件对试验数据进行回归分析。结果: 最佳提取工艺为加23倍量76%乙醇浸提2次

每次60 min。刺槐苷平均得率达1.834%

转移率86.88%。结论: 优选的工艺条件稳定可行

为刺槐花中刺槐苷的提取提供实验依据。

Abstract

Objective: To optimize extraction technology of robinin from flowers of Robinia pseudoacacia. Method: According to the design principle of Box-Benhnken

effects of ethanol volume fraction

liquid-material ratio

extraction time and extraction times on extraction rate of robinin were studied by response surface method

meanwhile regression analysis of these experimental data was used by SAS 9.2 software. Result: Optimum extraction conditions were obtained as follows:extracted 2 times with 23 times the amount of 76% ethanol

60 min per time.Under these conditions

the average extraction rate of robinin could be up to 1.834% with the transfer rate of 86.88%. Conclusion: These optimized conditions could provide experimental evidence for extracting of robinin from flowers of R. pseudoacacia.

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