Objective: To investigate in vitro transdermal permeability of Evodia rutaecarpa powder vinegar dressing in guinea pigs. Method: Modified Franz diffusion cell was adopted

with isolated guinea pig skin as transdermal barrier

through in vitro percutaneous absorption test

the content of evodiamine and rutaecarpine was determined by HPLC

then cumulative amount and permeation rate

in order to obtain penetration curve equation. Result: Transdermal absorption rate of evodiamine and rutaecarpine were 0.34

0.19 μg·cm-2·h-1

the cumulative amount of them in 24 h were 7.78

4.42 μg·cm-2. Conclusion: Evodiamine and rutaecarpine from E. rutaecarpa powder dressing vinegar had good in vitro transdermal absorption characteristics

it could provide a reference for study on percutaneous absorption preparation of E. rutaecarpa.