YU Zi-chuan, YU Jie-jing, HAN Jian-wei. Optimization of Extraction Technology for Total Saponins from by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 50-53.
YU Zi-chuan, YU Jie-jing, HAN Jian-wei. Optimization of Extraction Technology for Total Saponins from by Box-Behnken Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 50-53. DOI: 10.11653/syfj2013100050.
通过单因素试验和Box-Behnken效应面法考察加水量、提取时间、提取次数、浸泡时间等因素对提取工艺的影响。 结果: 最佳提取工艺为加8.8倍量水浸泡90 min
煎煮提取2次
每次2.5 h。地乌总皂苷提取率11.23%。 结论: 优选的提取工艺稳定可行
数学模型的预测值与试验观察值相符。
Abstract
Objective: To optimize extraction technology of total saponins from Anemone flaccida. Method: With extraction rate of total saponins as dependent variable
soaking time
the amount of water
extraction time as independent variables
effects of influencing factors on extraction technology was investigated by single factor test and Box-Behnken response surface methodology. Result: Optimum process parameters were obtained as followings:soaked 90 min with 8.8 times the amount of water
boiled 2 times with 2.5 hours per time.Extraction rate of total saponins was 11.23%. Conclusion: This optimized technology was stable and feasible
the predictive value of mathematical model and the experimental observation value was fitted.