Objective: To develop a RP-HPLC-DAD method for determination of forsythiaside B

poliumoside

verbascoside in the leaves and stems of Callicarpa kwangtungensis from different habitats. Method: The separation was performed on a Phenomenex Luna C18 column(4.6 mm×250 mm

5 μm) with a mobile phase of acetonitrile-0.1% acetic acid(20:80)

the flow rate was 1.0 mL·min-1

the column temperature was 30 ℃ and the detecting wavelength was at 330 nm. Result: The linear ranges of forsythiaside B

poliumoside and verbascoside were 10.1-505

9.96-498

20.8-1004 mg·L-1

respectively;The average recoveries of three kinds of benzene glycosides were 104.2%(RSD 0.71%)

101.3% (RSD 0.59%)

98.69% (RSD 1.1%)

respectively.Samples of C. kwangtungensis from different habitats were analyzed

the results showed that content range of forsythiaside B

poliumoside and verbascoside were 0.102%-1.59%

0.076%-1.87%

0%-3.19%

respectively. Conclusion: This established method was rapid

accurate and repeatable.It could be used to simultaneously determinate the content of forsythiaside B

poliumoside and verbascoside in C. kwangtungensis and provide experimental basis for quality control of C. kwangtungensis.