HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量

张海伟; 张艺; 杨继家; 范刚

doi:10.11653/syfj2013100095

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HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量

更新时间：2024-01-04

- HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量
- Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC
- 中国实验方剂学杂志 2013年19卷第10期页码：95-98
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30973957,30960507);高校博士点专项科研基金项目(20095132110008)
- DOI：10.11653/syfj2013100095
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- 纸质出版日期：2013
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张海伟, 张艺, 杨继家, 等. HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量[J]. 中国实验方剂学杂志, 2013,19(10):95-98.

ZHANG Hai-wei, ZHANG Yi, YANG Ji-jia, et al. Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 95-98.
张海伟, 张艺, 杨继家, 等. HPLC测定不同批次藏药三果汤中没食子酸和鞣花酸的含量[J]. 中国实验方剂学杂志, 2013,19(10):95-98. DOI： 10.11653/syfj2013100095.

ZHANG Hai-wei, ZHANG Yi, YANG Ji-jia, et al. Determination of Gallic Acid and Ellagic Acid from the Oxidation Resistance of Traditional Tibetan Drug Sanguotang of Different Batches by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 95-98. DOI： 10.11653/syfj2013100095.

摘要

目的: 建立同时测定藏药三果汤中抗氧化活性成分没食子酸和鞣花酸含量的方法

并对不同批次中的含量进行比较。方法: 采用HPLC

Welchrom C18色谱柱(4.60 mm×250 mm

5 μm)

流动相乙腈-0.25%甲酸溶液

梯度洗脱

流速0.85 mL·min-1

柱温25 ℃

检测波长260 nm。结果: 没食子酸和鞣花酸分别在0.050～0.700

0.050～0.714 μg呈良好的线性关系

平均回收率分别为100.57%

98.71%

RSD分别为0.84%

1.16%。结论: 所建方法操作简便、准确、重复性好

适用于同时测定藏药三果汤中没食子酸和鞣花酸的含量

含量测定结果为藏药三果汤的药用研究、产品的开发提供了依据。

Abstract

Objective: To determine the gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang by RP-HPLC and compare the content of different batches. Method: The separation was performed on a welchrom C18 (4.60 mm×250 mm

5 μm) column with gradient elution.The mobile phase was acetonitrile-0.25% methanoic acid solution

and the flow rate was 0.85 mL·min-1.The detection wavelength was set at 260 nm. The column temperature was kept at 25 ℃. Result: The linear ranges of gallic acid and ellagic acid were within 0.050 0-0.700 0

0.050 2-0.714 0 mg respectively. The average recoveries for gallic and ellagic acid were 103.22%

97.49% (n=6). Conclusion: This method is suitable for the determination of gallic acid and ellagic acid in the traditional Tibetan drug Sanguotang. The results provide a basis for medicinal value research and product development of the traditional Tibetan drug Sanguotang.

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