Objective: To establish the method of chromatography-AFS for separating and determining As from different existing forms in Bupleurum chinense. Method: Chromatography-AFS was used to determine the content of As. The conditions of chromatography and spectrum were as follows: negative ion column of PRP-X100 (4.1 mm×250 mm

10 μm) was used

mobile phase was the solution of 15 mmoL·L-1 (NH4)2HPO4 (pH 6.0). The flow rate was 1 mL·min-1; column temperature was set at 25 ℃; element lamp was hollow cathode lamp of As

lamp current/auxiliary cathode lamp current was 100/46 mA. Result: The standard curve was linear in the range of 0.002-0.01 μg. The average recovery was 97.35% with RSD 1.5% (n=6). Conclusion: The method is sensitive

accurate and reproducible. It can be used to determine the content of As in different existing forms.