HPLC测复方野菊花含片中黄芩苷和蒙花苷含量

陈红英; 倪健; 张辉

doi:10.11653/syfj2013100122

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HPLC测复方野菊花含片中黄芩苷和蒙花苷含量

更新时间：2024-01-04

- HPLC测复方野菊花含片中黄芩苷和蒙花苷含量
- Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC
- 中国实验方剂学杂志 2013年19卷第10期页码：122-124
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- DOI：10.11653/syfj2013100122
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- 纸质出版日期：2013
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陈红英, 倪健, 张辉. HPLC测复方野菊花含片中黄芩苷和蒙花苷含量[J]. 中国实验方剂学杂志, 2013,19(10):122-124.

CHEN Hong-ying, NI Jian, ZHANG Hui. Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 122-124.
陈红英, 倪健, 张辉. HPLC测复方野菊花含片中黄芩苷和蒙花苷含量[J]. 中国实验方剂学杂志, 2013,19(10):122-124. DOI： 10.11653/syfj2013100122.

CHEN Hong-ying, NI Jian, ZHANG Hui. Simultaneous Determination of Baicalin and Buddleoside in Compound Yejuhua Buccal Tablets by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 122-124. DOI： 10.11653/syfj2013100122.

摘要

目的: 建立复方野菊花含片中黄芩苷和蒙花苷含量测定的方法。方法: 采用HPLC

Kromasil-C18(4.6 mm×250 mm

5 μm) 色谱柱

流动相乙腈-0.1%磷酸(25:75)

检测波长334 nm。结果: 黄芩苷和蒙花苷进样量分别在0.460～2.760 μg (r=0.999 5)

0.020～0.400 μg (r=0.999 9)线性关系良好;黄芩苷和蒙花苷平均回收率分别为101.77% (RSD 0.98%)

98.36% (RSD 1.68%)。结论: 该方法简便易行

重复性好

可用于复方野菊花含片中黄芩苷和蒙花苷含量测定。

Abstract

Objective: To establish a RP-HPLC method for simultaneous determination of baicalin and buddleoside in Compound Yejuhua Buccal Tablets. Method: HPLC was adopted to determine the content of baicalin and buddleoside. The Kromasil-C18 Column (4.6 mm×250 mm

5 μm) was used with the mobile phase consisting of acetonitrile-0.1% aqueous phosphoric acid (25:75); the detection wavelength was set at 334 nm. Result: There was good linearity between the peak area and concentration within the range of 0.460-2.760 μg (r=0.999 9) for baicalin and 0.020-0.400 μg (r=0.999 9) for buddleoside respectively. The average recoveries of baicalin and buddleoside were 101.77% (RSD 0.98%) and 98.36% (RSD 1.68%) respectively (n=6). Conclusion: The method is simple

accurate and specific for quality control of Compound Yejuhua Buccal Tablets.

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