Objective: To establish an HPLC method for the study of tissue distribution of compound Salvia plebeian granules in rats and provide theoretical basis for its clinical application. Method: The biological samples were extracted with acetonitrile

the Agilent TC-C18 (4.6 mm×250 mm

5 μm) column was used as analytical column with a mobile phase consisted of methanol and water(55: 45) at a flow rate of 1.0 mL·min-1. Result: The content of homoplantaginin was highest in stomach

and the concentration was (7.496±0.636) μg·g-1 after administration 1 hour later

then decreased to (5.237±0.373) μg·g-1 and (1.763±0.157) μg·g-1 after 4 h

7 h. Followed by kidney and small intestine

the content in heart

spleen and lung was relatively low. Conclusion: The homoplantaginin was distributed in all organs of rats after administrated compound S. plebeian granules. The higher distribution of homoplantaginin in kidney can be explained by the forming theory of urinary tract infection.