Objective: To investigate effect of Jiawei Sijunzi decoction containing serum on B16 malignant melanoma cell proliferation and apoptosis. Method: Jiawei Sijunzi decoction was administrated by serum pharmacological method

which B16 melanoma cells were cultured in 37 ℃ and 5% CO2 thermostat incubator

and logarithmic phase cells were collected and exposed to final concentration 10% mice containing serum in 12

24

48 h

respectively.The experimental group were divided into blank control group

serum control group

traditional Chinese medicine group (high dose

middle dose and low dose)

and cisplatin group

and each group contained 5 samples. MTT and Flow cytometry Annexin V-FITC/PI assay were used to analyse the Jiawei Sijunzi decoction containing serum influence on B16 malignant melanoma cell proliferation and apoptosis respectively. Result: A time-dependent increase in inhibiting B16 malignant melanoma cell proliferation by Jiawei Sijunzi decoction containing serum was observed compared to blank control group

serum control group in the same time period

and middle dose traditional Chinese medicine group inhibition rate was 50.22% in 12 h and 53.81% in 24 h respectively

and reached the half of inhibition rate

and half of inhibition rate in 48 hours period and 24 hours period were compared to no significant change. Middle dose traditional Chinese medicine group

high dose traditional Chinese medicine group and cisplatin group (5 mg·L-1) apoptosis rate were (19.70±0.98)%

(25.21±1.03)%

(21.31±1.00)% respectively

and promote significantly B16 malignant melanoma cell apoptosis compared to the blank control group and serum control group

the rate of cisplatin group apoptosis is high than middle dose traditional Chinese medicine group

and lower than high dose traditional Chinese medicine group. Conclusion: Jiawei Sijunzi decoction containing serum can inhibit the proliferation of B16 malignant melanoma cells

and promote the apoptosis of B16 malignant melanoma cells

which could be the therepy mechanism of Jiawei Sijunzi decoction antitumor.