Objective: To observe the effect of breviscapine on renal tissue vasoactive substances and to analyze the protective mechanism of breviscapine on renal tissue in obstructive nephropathy rat. Method: According to the weight the SD rats were randomly divided into 5 groups

sham operation group(SOG)

obstructive nephropathy model group(MG)

benazepril group(BG)

100 mg·kg-1 breviscapine group(100 mg-BVG) and 200 mg·kg-1 breviscapine group(200 mg-BVG). BG was given benazepril 5 mg·kg-1 by gavage (ig)

100 mg-BVG and 200-mgBVG were given breviscapine 100 mg·kg-1 and 200 mg·kg-1 gavage

SOG and ONMG were treated with 0.9% sodium chloride solution by gavage

1/day. The administration lasted 7 days. After administration the rat kidneys were weighed and NAG-U were measured

left renal histopathology were observed by HE and Masson staining; the expression of α-SMA was detected by immunohistochemistry; angiotensin Ⅱ(Ang Ⅱ)

angiotensin converting enzyme (ACE)

nitric oxide (NO) and endothelin (ET-1) content change were measured by radioimmunoassay and colorimetric determination. Result: Compared with the SOG

ONMG rats NAG-U and left renal weight were significantly increased(P<0.01)

and a large amount of inflammatory cell infiltration and hyperaemia had showed

ET-1

ACE and Ang Ⅱ were obviously increased but the content of NO were significantly decreased(P<0.01). Compared with the ONMG

in the BG

100 mg-BVG and 200 mg-BVG NAG-U and the left renal weight were reduced (P<0.05

P<0.01)

inflammatory cell infiltration and hyperaemia were also reduced. Compared with the ONMG

the two BVGs ET-1

ACE and Ang Ⅱcontent were decreased but NO were increased(P<0.05

P<0.01). Conclusion: Breviscapine probably alleviate obstructive nephyropathy by affecting the vasoactive substances

downregulation of ET-1

ACE and Ang Ⅱcontent

the upregulation of NO.