B1和B10细胞在溃疡性结肠炎患者外周血中的表达及黄芩苷对其体外表达的影响

冯锦山; 王士群; 叶营; 周小戈; 郑学宝

doi:10.11653/syfj2013100245

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B1和B10细胞在溃疡性结肠炎患者外周血中的表达及黄芩苷对其体外表达的影响

更新时间：2024-01-04

- B1和B10细胞在溃疡性结肠炎患者外周血中的表达及黄芩苷对其体外表达的影响
- Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression
- 中国实验方剂学杂志 2013年19卷第10期页码：245-248
- 作者机构：
- 作者简介：
- 基金信息：
  
  广东省自然科学基金项目(10152402301000003)
- DOI：10.11653/syfj2013100245
  中图分类号：
- 纸质出版日期：2013
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冯锦山, 王士群, 叶营, 等. B1和B10细胞在溃疡性结肠炎患者外周血中的表达及黄芩苷对其体外表达的影响[J]. 中国实验方剂学杂志, 2013,19(10):245-248.

FENG Jin-shan, WANG Shi-qun, YE Ying, et al. Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 245-248.
冯锦山, 王士群, 叶营, 等. B1和B10细胞在溃疡性结肠炎患者外周血中的表达及黄芩苷对其体外表达的影响[J]. 中国实验方剂学杂志, 2013,19(10):245-248. DOI： 10.11653/syfj2013100245.

FENG Jin-shan, WANG Shi-qun, YE Ying, et al. Expression of B1 and B10 Cells in Peripheral Blood of Patients with Ulcerative Colitis and Baicalin Effect on its Expression [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 245-248. DOI： 10.11653/syfj2013100245.

摘要

目的: 检测与分析溃疡性结肠炎(UC)患者外周血CD19+CD5+B细胞(B1)及CD19+CD5+CD1d+B细胞(B10)的比例

探讨黄芩苷对其体外表达的影响。方法: 收集并分离活动期UC患者其外周血单核细胞(PBMC)

以年龄性别相匹配的正常人PBMC为对照

经脂多糖(LPS)+黄芩苷体外刺激淋巴细胞

流式细胞术检测刺激前后外周血B1和B10细胞比例。结果: UC患者外周血中B1和B10细胞占CD19+B细胞的比例(4.83%±3.26%

0.97%±0.48%)低于正常对照组(16.16%±11.89%

5.99%±3.59%)

P<0.05

LPS+黄芩苷体外刺激能上调UC患者B10淋巴细胞的比例(P=0.02)

但对正常人该淋巴细胞亚群未见明显影响。结论: B1和B10细胞可能在UC病程中发挥免疫调节作用

黄芩苷可能对UC患者的B10细胞有上调作用。

Abstract

Objective: The detection and analysis the proportion of CD19+CD5+B cells (B1) and CD19+CD5+CD1d+B cells (B10) in peripheral blood of patients with ulcerative colitis (UC)

exploring the effect of baicalin on up-regulating both the two subpopulations expression in vitro. Method: Separate peripheral blood mononuclear cells (PBMC) of UC patients

using the PBMCs of age and gender-matched helthy people as normal control

lymphocytes were stimulated by lipopolysaccharide(LPS)+baicalin in vitro

B1 and B10 cells proportion were detected by the flow cytometry before and after stimulation. Result: The proportion of B1 and B10 cells are decrease in UC patients peripheral blood compare to the normal control group(P=0.029

0.049). In vitro

LPS+baicalin stimulation raised the proportion of B10 lymphocytes of UC patients(P=0.02)

but no significant impact on normal subjects. Conclusion: UC may be related to the expression of B1 and B10 cells

baicalin might upregulates the proportion of B10 cells of UC patients.

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