Objective: To establish a mouse model with kidney Yang deficiency infected by influenza virus. Method: Kidney Yang deficiency mice were established by intraperitoneal injection of estradiol benzoate (0.008 mg·g-1) as previously described. The physical signs in mice were observed

and the swimming time of mice

rectal temperature

toe temperature

autonomic mobility were examined.On the basis of above model

Mice were inoculated with mouse lung influenza virus (FM1) by the nasal cavity to establish the mice model with kidney Yang deficiency infected by influenza virus. Then physical signs

mortality

body weight and temperature changes were observed daily.The concentrations of tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in mouse serum were measured

and the organ indexes of lung

spleen

kidney

adrenal and thymus were detected. Pathological changes of lung were observed. Result: The swimming time

rectal temperature

toe temperature

and autonomic activities of mice model were significantly decreased compared with normal group (P<0.01); Viral pneumonia was detected in lung tissue. Compared with the normal control

the thymus index of model mice was remarkably reduced (P<0.01). The level of IFN-γ was obviously increased (P<0.05) and TNF-α level was decreased. Conclusion: To some extent

the symptoms of kidney-yang deficiency with influenza disease could embodied in this mouse model

which was successfully established by combining estradiol intraperitoneal injection with nasal cavity by influenza virus FM1 and this provided a more ideal experimental model for pathogenesis and drug intervention.