WEI Wei, XIANG Fang, XIANG Hong. Effects of Elemene Injection on Proliferation and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE1[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 283-285.
WEI Wei, XIANG Fang, XIANG Hong. Effects of Elemene Injection on Proliferation and Apoptosis of Nasopharyngeal Carcinoma Cell Line CNE1[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(10): 283-285. DOI: 10.11653/syfj2013100283.
Objective: To study the effect of elemene injection on proliferation and apoptosis of nasopharyngeal carcinoma cell line CNE1. Method: The cytotoxic effect of elemene injection (20
10
5
2.5
0 g·L-1: 12
24
48 h) on CNE1 cells was tested by Prestoblue
cell morphological changes was observed by light microscope and apoptosis of CNE1 cells was observed by Hoechst-PI double staining and flow cytometric analysis. Result: Elemene injection showed an inhibitory effect with dose-response relation on CNE1 cells. The IC50 values at 12
24
48 h were (10.70±0.26)
(7.06±0.11)
(5.52±0.10) g·L-1 respectively.Light microscope and hoechst-PI double staining showed apoptotic morphology change. Conclusion: Elemene injection can inhibit the proliferation of cell line CNE1 through the induction of apoptosis.