Objective: To investigate the regulated renal gene expression prolife of cyclovirobuxine-D(CVB-D) in rats

and explore its mechanism of renal toxicity. Method: Ten SD rats were divided into 2 group randomly: normal group and CVB-D 10 mg·kg-1 group.Rats were continuous gavaged 14 days.The total RNAs were isolated and purified from kidney of normal and CVB-D 10 mg·kg-1 rats. Both the mRNAs from two kinds of tissues were reversely transcribed to cDNA with the incorporation of fluorescent labeled hybridization probes. The mixed probes were hybridized to cDNA chip

and then were screened through the analysis of fluorescent intensity.RT-PCR was used to confimed the results of 4 choosing gene chip. Result: Compared with the control group

gene expression of 309 were changed

147 gene expression up-regulated

and 162 gene expression down-regulated. All of the gene changes were related with apoptotic signal

MAP Kinase cascade

calcium-mediated signaling

and cell cycle proteins. Conclusion: The renal toxicity of CVB-D involved in regulating specific genes abnormal expression including apoptosis

MAP Kinase cascade

Calcium-mediated signaling and cell cycle protein

etc.