蜣螂中间体的大孔树脂纯化工艺优选及检测

谭承佳; 马家骅; 蒋巧梅; 杨明

doi:10.11653/syfj2013110008

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蜣螂中间体的大孔树脂纯化工艺优选及检测

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- 蜣螂中间体的大孔树脂纯化工艺优选及检测
- Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin
- 中国实验方剂学杂志 2013年19卷第11期页码：8-10
- 作者机构：
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(30902005);绵阳市科技计划项目(11F020)
- DOI：10.11653/syfj2013110008
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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谭承佳, 马家骅, 蒋巧梅, 等. 蜣螂中间体的大孔树脂纯化工艺优选及检测[J]. 中国实验方剂学杂志, 2013,19(11):8-10.

TAN Cheng-jia, MA Jia-hua, JIANG Qiao-mei, et al. Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 8-10.
谭承佳, 马家骅, 蒋巧梅, 等. 蜣螂中间体的大孔树脂纯化工艺优选及检测[J]. 中国实验方剂学杂志, 2013,19(11):8-10. DOI： 10.11653/syfj2013110008.

TAN Cheng-jia, MA Jia-hua, JIANG Qiao-mei, et al. Optimization and Detection of Purification Technology for Intermediates of by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 8-10. DOI： 10.11653/syfj2013110008.

摘要

目的: 优选蜣螂提取物的DA201-C型大孔树脂纯化工艺。方法: 采用单因素试验考察DA201-C型树脂的吸附性能和影响纯化工艺的pH、洗脱剂、上柱流速等因素;采用氨基酸自动分析仪检测纯化后的中间体。结果: 最佳纯化工艺条件为上样液质量浓度17.60 g·L-1

吸附流速2 BV·h-1

pH 4.0

依次用1 BV水和4 BV 70%乙醇洗脱

脱盐率达98.81%

多肽回收率达87.39%

所得醇洗脱物含结合型氨基酸(多肽)32.26%。结论: 该工艺分离效果较好

可用于蜣螂中间体的脱盐与纯化。

Abstract

Objective: To optimize purification technology of Catharsius molossus extract by DA201-C macroporous resin. Method: Single factor test was adopted to evaluate absorption capacity of DA201-C macroporous resin and some factors affecting purification technology

such as pH

eluting agent

flow rate and other factors.Automatic amino acid analyzer was used to analyze purified intermediates. Result: The best purification technology were as follows:the concentration of the sample solution 17.6 g·L-1

adsorption rate 2 BV·h-1

pH of the sample solution 4.0

eluted by 1 BV of deionized water and 4 BV of 70% ethanol

respectively.Desalination rate was up to 98.81%

the recovery rate of peptides in intermediates of C. molossus was up to 87.39%

the total content of bound amino acids (polypeptides) in alcohol eluate was 32.26%. Conclusion: This technology was simple

feasible and could be used for desalination and purification of intermediates of C. molossus.

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