Objective: To optimize purification technology of Kubai Fuyan suppositories. Method: Macroporous resin method was adopted

with the content of berberine hydrochloride

matrine and osthole as index

type of macroporous resin and its purification technology was screened by single factor test.The content of indicators ingredients were determined by HPLC. Result: HPD-400A type macroporous resin was selected with optimum purification technology was as following:ratio of resin and crude drug 1: 3

the sample solution concentration of g·mL-1 with pH 8

elution flow rate of 1.5 BV·h-1

eluated with 5 BV water

then eluated with 5 BV of 20% ethanol and 40% ethanol

merged ethanol eluent

recovered

dried

then received purified extracts. Conclusion: Drug loading of suppositories increased 4.3 times after purified with HPD-400A type macroporous resin.This optimized purification technology was stable and feasible

which could be used for industrial production of Kubai Fuyan suppositories.