LI Jian, ZHAO Ting, LIN Hui, et al. Optimization of Extraction Technology of Total Saponins from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 45-47.
LI Jian, ZHAO Ting, LIN Hui, et al. Optimization of Extraction Technology of Total Saponins from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 45-47. DOI: 10.11653/syfj2013110045.
Objective: To optimize extraction process of total saponins from Mirabilis himalaica. Method: Taking Ginsenoside Re as a reference substance
the content of total saponins was determined by vanillin-perchloric acid colorimetric method.Effect of ethanol concentration
extraction temperature
extraction time
extraction times and solid-liquid ratio on yield of total saponins was investigated by single factor test and orthogonal test. Result: Effect of factors on yield of total saponins was in order of extraction temperature>extraction time>solvent concentration.Optimum process was as following:extracted 2 times with 12 folds of 60% ethanol at 70 ℃
2 h per time. Yield of total saponins was 0.704%. Conclusion: It is feasible that total saponins from M. himalaica was extracted by reflux method.