Objective: To optimize conventional

microwave and ultrasonicwave-assisted vacuum reflux extraction technologies for total from Epimedium brevicornu

and compare extraction efficiency of this 3 technologies. Method: Taking yield of icariin as index

L9(34) orthogonal test was used to optimize conventional

microwave and ultrasonicwave-assisted vacuum reflux extraction technologies. Result: Optimum technology conditions of conventional vacuum reflux extraction were as followings:vacuum degree 0.07 MPa

extracted 3 times with 20 minutes each time

yield of icariin 88.06%.Optimum conditions of microwave-assisted vacuum reflux extraction technology were:extraction power 500 W

extracted 2 times with 20 minutes per time

extraction rate of icariin was 93.27%.Optimum conditions of ultrasonicwave-assisted vacuum extraction technology were:extraction power 200 W

extracted 2 times with 10 minutes per time

extraction rate of icariin was 94.16%. Conclusion: These three vacuum reflux processes were all better than atmospheric reflux extraction.In practical application

we should choose process with high efficiency

little pollution

energy saving

convenient operation.