Objective: To get anti-hypertensive activity polypeptides from porcine blood. Method: Through in vitro simulation of in vivo metabolism

enzymatic hydrolysis

ultrafiltration and gel column chromatography were adopted to separate polypeptides

and MALDI-TOF-MS was used to identify structures.Inhibition of anti-hypertensive activity polypeptides from porcine blood to angiotensin-converting enzyme (ACE) was detected

and in vitro activity tracking adopted;Effect of these activity polypeptides on systolic blood pressure (SBP) of spontaneously hypertensive rats (SHR) was detected

and adopted in vivo activity verification. Result: An active polypeptide pep4 was obtained from porcine blood

its amino sequence was VVLLGDV with relative molecular mass of 714 Da

it had a strong in vitro ACE inhibitory activity with IC50=0.325 g·L-1.During 1-36 h after administration

pep4 had a significant reduction(P<0.05) in blood pressure on SHR and SBP decreased 10-25 mmHg

antihypertensive effect after administrating 15 h was the most obvious. Conclusion: Polypeptide pep4 was first obtained from porcine blood

it had a significant antihypertensive effect.As a active peptide source for looking for antihypertensive activity peptides

it had prospects for development of new antihypertensive drugs

and it was worth of further study.