Objective: To establish HPLC-ELSD characteristic fingerprint for Ermu granules. Method: HPLC-ELSD was employed

separation was carried out on Global Chromatography C18column (4.6 mm×250 mm

5 μm)

mobile phase of water-acetonitrile with gradient elution

flow rate 1.0 mL·min-1

temperature of drift tube maintained at 45 ℃

the gain value 1

flow rate of atomizing air set at 1.5 L·min-1.Ten batches of Ermu granules were determined

and fingerprints of them were compared with raw materials of Anemarrhena asphodeloides and extracts of A. asphodeloides

similarity among them was analysed by 2004 Edition A of 'Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine'. Result: The similarity between ten batches of Ermu granules and raw materials of A. asphodeloides

extracts of A. asphodeloides were very high(0.960-0.998). Conclusion: HPLC-ELSD fingerprint of Ermu granules could make its quality evaluation system more completed

which could provide a reference for study of 'raw materials of Chinese medicine-intermediate materials-products' for the whole process control of other preparations varieties.