HPLC测定糙枝金丝桃中绿原酸、芦丁、金丝桃苷、槲皮素和山奈酚

郭炬亮; 李新霞; 支玲

doi:10.11653/syfj2013110075

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HPLC测定糙枝金丝桃中绿原酸、芦丁、金丝桃苷、槲皮素和山奈酚

更新时间：2024-01-04

- HPLC测定糙枝金丝桃中绿原酸、芦丁、金丝桃苷、槲皮素和山奈酚
- Simultaneous Determination of Chlorogenic Acid, Rutin, Hyperoside, Quercetin and Kaempferol in by HPLC
- 中国实验方剂学杂志 2013年19卷第11期页码：75-78
- 作者机构：
- 作者简介：
- 基金信息：
  
  新疆医科大学科研创新基金项目(XJC201114)
- DOI：10.11653/syfj2013110075
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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郭炬亮, 李新霞, 支玲. HPLC测定糙枝金丝桃中绿原酸、芦丁、金丝桃苷、槲皮素和山奈酚[J]. 中国实验方剂学杂志, 2013,19(11):75-78.

GUO Ju-liang, LI Xin-xia, ZHI Ling. Simultaneous Determination of Chlorogenic Acid, Rutin, Hyperoside, Quercetin and Kaempferol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 75-78.
郭炬亮, 李新霞, 支玲. HPLC测定糙枝金丝桃中绿原酸、芦丁、金丝桃苷、槲皮素和山奈酚[J]. 中国实验方剂学杂志, 2013,19(11):75-78. DOI： 10.11653/syfj2013110075.

GUO Ju-liang, LI Xin-xia, ZHI Ling. Simultaneous Determination of Chlorogenic Acid, Rutin, Hyperoside, Quercetin and Kaempferol in by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 75-78. DOI： 10.11653/syfj2013110075.

摘要

目的: 建立高效液相色谱法同时测定糙枝金丝桃药材中绿原酸、芦丁、金丝桃苷、槲皮素、山奈酚的分析方法。方法: 用Sunfire C18(4.6 mm×250 mm

5 μm)色谱柱分离;乙腈-0.1%磷酸进行梯度洗脱;流速1.0 mL·min-1;检测波长360 nm。结果: 绿原酸、芦丁、金丝桃苷、槲皮素、山奈酚分别在0.01~0.61 μg(r=0.999 6)

0.10~2.02 μg(r=0.999 7)

0.10~1.01 μg(r=0.999 9)

0.10~1.24 μg(r=0.999 7)

0.005 7~0.17 μg(r=0.999 7)线性关系良好;平均加样回收率分别为98.87%(RSD 1.43%)

99.81%(RSD 0.86%)

99.98%(RSD 0.82%)

98.8%(RSD 1.73%)

99.85(1.79%)。结论: 该方法简便、准确

重复性好

可用于糙枝金丝桃药材的质量控制。

Abstract

Objective: To establish an HPLC method for the simultaneous determination of chlorogenic acid

rutin

hyperoside

quercetin and kaempferol in Hypericum scabrum. Method: A high-performance liquid chromatography equipped a Sunfire C18 (4.6 mm×250 mm

5 μm) column with UV detector used. The mobile phase consisted of acetonitrile and 0.1% aqueous phosphoric acid with gradient elution. The detection wavelength was set at 360 nm and the flow rate was 1.0 mL·min-1. Result: The linear ranges of chlorogenic acid

rutin

hyperoside

quercetin and kaempferol were 0.01-0.61 μg (r=0.999 6)

0.10-2.02 μg (r=0.999 7)

0.10-1.01 μg (r=0.999 9)

0.10-1.24 μg (r=0.999 7)

0.005 7-0.17 μg (r=0.999 7)

the average recoveries were 98.87% (RSD 1.43%)

99.81% (RSD 0.86%)

99.98% (RSD 0.82%)

98.8% (RSD 1.73%)

99.85 (RSD 1.79%). Conclusion: The method is simple

accurate and repeatable

which can be used for quality control of H. scabrum.

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