Objective: To develop an HPLC method for determination of six ingredients protocatechuic aldehyde

hyperoside

rosmarinic acid

sithospermic acid

salvianolic acid B

quercetin in hawthorn and salvia miltiorrhiza extracts. Method: The separation was performed on a Phenomsil-C18 ODS (4.6 mm×250 mm

5 μm) column with the gradient elution of acetonitrile-water of 0.5% phosphoric acid at the flow rate of 1.0 mL·min-1. Detection wavelength was set at 300 nm. The column temperature was maintained at 30 ℃. Result: Protocatechuic aldehyde

hyperoside

rosmarinic acid

lithospermic acid

salvianolic acid B

quercetin had good linearity in the ranges of 0.021 58-0.107 9 μg (r=0.999 4)

0.102 6-0.513 0 μg(r=0.999 2)

0.157 5-0.787 5 μg (r=0.999 0)

0.245 6-1.228 μg (r=0.999 1)

2.600-13.00 μg (r=0.999 0)

0.085 1-0.425 6 μg (r=0.999 1) respectively. The average recoveries (n=6) of protocatechuic aldehyde

hyperoside

rosmarinic acid

lithospermic acid

salvianolic acid B

quercetin were 99.1%

99.3%

100.2%

99.2%

98.9%

99.2% respectively with RSDs of the recovery were less than 3%. Conclusion: The developed method is helpful to control the quality of hawthorn and salvia miltiorrhiza extracts.