Objective: High-performance liquid chromatography array mass spectrometry (HPLC-MS) method was developed for determination of the concentration of 10 different ginsenosides of different medical parts of Panax quinquefolium

which collected from original habitat in Ontario of Canada. Method: Sample solution was separated on a DIKMA diamonsil (4.6 mm×250 mm

5 μm). Acetonitrile-0.05% phosphoric acid aqueous solution and acetonitrile-0.05% acetic acid aqueous solution were used as mobile phase and the flow rate through the HPLC column was 0.3 mL·min-1 and the entire effluent was directed to the mass spectrometer. The column temperature was kept at 35 ℃ and the UV detection wavelength was set at 203 nm. MS analysis was monitored in positive mode. The conditions of ESI source were as follows: sheath gas flow rate

10 L·min-1; sweep gas flow rate

10 L·min-1; spray voltage

4.5 kV; capillary temperature

320 ℃; capillary voltage 30 V. Result: All calibration curves showed good linearity (r>0.999) within the test ranges. The average recovery of the method was between 95% and 102%

RSD<2.68%. The concentration of single ginsenoside of root

stem and leaf was quite different; that of Re and Rb1 of root and that of Rb2 and Rb3 of leaf was higher than others; that of stem was lower than that of root and leaf

moreover that of some samples of leaf was higher than that of root. Conclusion: The method is simple

accurate

replicate and suitable for the determination of P. quinquefolium. It is suggested that the leaf of P. quinquefolium can be used a new resource of ginsenoside.