Objective: Effect of Zuogui Wan on the expression of c-Jun amino-terminal kinase(JNK) and c-Jun in rats with gentamicin-induced renal tubular injury. Method: Forty male Wistar rats were divided randomly into control group(C)

model group(M)

Sp600125 group(S) and Zuoguiwan group(Z). The C group was given ip injection of 0.9% saline 2.5 mL·kg-1·d-1

M group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1

S group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1 and ip injection of SP600125 15 mg·kg-1·d-1 Zuoguiwan group was given ip injection of gentamicin sulfate 100 mg·kg-1·d-1 and gavage Zuoguiwan aqueous solution 10 g·kg-1·d-1. Four groups of rats administered for 10 consecutive days. After the experiment measuring the NAG of rat. Hematoxylin-eosin to detect renal histopathology. Immunohistochemical techniques to detect JNK and c-Jun's expression of the rat kidney. Western blot techniques to detect JNK and c-Jun's expression of the rat kidney. Result: The results of biochemical index show: compared with the C group

the rats of M group NAG significantly increased

with a statistically significant difference (P<0.01); compared with the M group

the biochemical parameters improved of S group and Z group

with a statistically significant difference (P<0.05). The HE staining results show: the C group of renal tubule

renal interstitial no change; the M group of renal tubular epithelial cells swelling

vacuolar degeneration. The S group and Z group renal tubule epithelial cells slight swelling

degeneration. The immunohistochemical results show: compared with the C group

the rats of M group JNK

c-Jun abundantly expressed in the renal tubules

with a statistically significant difference (P<0.01); compared with the M group

protein expression was not significant in the S group and Z group

with a statistically significant difference (P<0.01). The results of western blot show: compared with the C group

the rats of M group JNK

c-Jun's gray values increased significantly

with a statistically significant difference (P<0.01); compared with the M group

the gray values of S group and Z group decreased

with a statistically significant difference (P<0.05

P<0.01). Conclusion: The protective effect of Zuogui Wan relieved the gentamicin-induced renal tubular injury in rats

which mechanism may be involved in the inhibition of the c-Jun N-terminal kinase activation.