Objective: To investigate the inhibition effects of Xiancidan(XCD) on the lung-cancer cell in Lewis tumor-bearing mice and explore its mechanism. Method: C57BL/6 mice were taken as model

and divided into model group

cis-platinum group

high dose group XCD

middle dose group XCD

low dose group XCD

combination of XCD and control group 5 days later

All groups were administrated for 13 days

and the respective dosage was 0

2.0(ip)

0.648(ig)

0.324(ig)

0.162(ig)

0.162(ig)+2.0(ip).To observe the effect of XCD on inhibition of the lung cancer cell and immune regulation

AnnexinⅤ-FITC/PI method was applied to determine the apoptosis and cycle of transplanted cancer cells and Western blot method was used to observe the p53

Bcl-2 gene expression. Result: ①The inhibition rate in positive control group

high dose group

middle dose group

low dose groupof XCD

combination of XCD and control group were(63.02±3.21)%

(46.59±2.60)%

(42.48±2.77)%

(15.74±1.94)%

(64.56±1.62)%.②The pleen Index of high dose group XCD

middle dose group XCD and low dose group were significantly different from model group(P<0.01). ③The apoptosis rate of model group

control group

high dose group XCD

middle dose group XCD

low dose group XCD

the combination group of XCD and positive drug was 0.04%

30.40%

14.80%

17.98%

16.04%

20.34%

respectively.④Compared with model group

the percentage of G0/G1 cell in positive control group

high dose group XCD

middle dose group XCD and the combination groupm was significantly decreased. ⑤The p53 expression and Bcl-2 rate was significantly different from model group. Conclusion: The XCD effective parts can inhibit the growth of lewis tumor

which is related to its apoptosis-inducing and immune regulation effect.