Objective: To explore the effect of Mori Folium total flavonoid (MFTF) on β-cells in rats with type-2 diabetic mellitus (T2DM). Method: The rat T2DM model was replicated by streptozotocin(STZ

30 mg·kg-1 body weight) injection

the modeling rats were randomly divided into five group

the model group

the MFTF (50

100

150 mg·kg-1)groups and rosiglitazone (1.8 mg·kg-1)group. 10 normal rats were taken control group. The treatment groups were orally administered once per day

model group was orally administered with distilled water.After treatment for 5 week

fasting blood glucose(FBG) and insulin(FINS) level in serum were estimated. Malondialdehyde (MDA) content

superoxide dismutase(SOD) and glutathione peroxidase (GSH-Px) activity in pancreatic tissue were measured. The expression of Bcl-2/Bax in islet tissue was examined by using immunohistochemistry. The pancreatic islet β cell apoptosis was detected by TUNEL. Result: MFTF could decrease MDA level significantly and increase SOD

GSH-Px level in pancreatic tissue (P<0.05-P<0.01)

increase the levels of Bcl-2 expression and decrease the expression of Bax protein significantly(P<0.05-P<0.01). Decrease pancreatic β-cell apoptosis(P<0.05-P<0.01)

decrease the level of glucose

insulin and insulin resistance index(P<0.05-P<0.01)

especiallythe high dose group (150 mg·kg-1) showed a best action. Conclusion: MFTF can improve insulin resistance and resists oxidative stress reaction and decrease apoptosis of pancreatic islet β cell.