Objective: To study the anti-fatigue effect of flavonoids from Mori Folium and discuss its mechanism. Method: Male Kunming mice were randomly divided into groups of flavonoids group (0.4

1g·kg-1 mulberry leaves flavonoids

respectively)

creatine group (creatine 3 g·kg-1)

control and normal group (stomach irritation with saline only). The exhaustive swimming experiment was performed 14 days later. The time of exhaustive swimming of mice was recorded and chemistry parameters correlated with fatigue was determined. The effects of flavones of Mori Folium on scavenging free radicals and anti-oxidation were investigated in vitro. Result: The time of exhaustive swimming of flavonoids groups and creatine group was longer than that of control group (P<0.05). The content of the creatinine and urea nitrogen level of serum decreased significantly the MDA production in liver tissues of mice in flavonoids group is lower than that of control group (P<0.05). Conclusion: Flavonoids from Mori Folium can improve the anti-fatigue capacity obviously and has a significant effect on scavenging free-radicals. The anti-fatigue effect of total flavonoids from Mori Folium may have correction with its radicals scavenging effects.