Objective: To observe the influence of extract from Rhizoma Phragmitis on male rats renal calcium oxalate. Method: Forty male SD rats were randomly divided into five groups(n=8 each):control

model

and Rhizoma Phragmitis intervention groups. After 4 weeks

the 24 h urine concentration oxalate(Ox)

Ca2+

the concentration of serum blood urea nitrogen(BUN)

creatinine(Cr)

Ca2+

P

and malondialdehyde(MDA)

superoxide dismutase(SOD) of renal tissue were detected. The calcium oxalate deposit in the kidney was observed by microscopy. Immunohistochemistry were used to assess the protein expression of osteopontin(OPN) in rat renal tissue of every groups. Result: The 24 h urine concentration Ox

Ca2+ and serum Cr of Rhizoma Phragmitis intervention group was lower than stone formation group obviously(P<0.05).rhizoma phragmitis intervention group the enzyme activity of SOD of renal tissue was higher than stone formation group(P<0.05). Stone formation group concentration of MDA of renal tissue was higher than Rhizoma Phragmitis intervention group(P<0.05).In normal control group

only a weak OPN protein staining was found. The expression was enhanced in the stone group. The extract from Rhizoma Phragmitis-treated rats tended to have lower OPN protein expression than the stone group. Conclusion: The extracts from rhizoma phragmitis can block the formation of renal calcium oxalate stone in rats potently.