Objective: To investigate the influence of Qingying Jiebiao mixture (QJM) on immune function in mice infected by flu virus. Method: The model of influenza virus in mice was established by lung infection with Asia line(A/PR/8/H1N1). QJM suspension (0.8 g·mL-1) 0.4 mL was orally given to each mouse (16 g·kg-1) daily for 12 days. After administration of 3

6

9 d and 12 d

the expression level of specific cytokine interferon-gamma

tumor necrosis factor-α

interleukin 3

4

6

10

the ratio of Th1 (interferin-γ

IFN-γ) and Th2 (interleukin-4

IL-4) were determined by flowcytometry. Result: After the mice was infected with influenza virus

cytokines IFN-γ at 3 d was increased significantly (P<0.05)

those at 6

9 d and 12 d showed no significant changes. IFN-γ at 6

9 d was decreased significantly compared with that at 3 d (P<0.05); and cytokine tumar necrosis factoi-α(TNF-α) at 3

6

9

12 d were increased significantly (P<0.01

P<0.05); cytokines IL-3 at 3

6

9

12 d was increased

those at 3

6 d showed significant difference (P<0.05). Cytokines IL-4

IL-6 and IL-10 at 3

6

9

12 d were significantly increased (P<0.01)

and reached the peak in the early 3 d. Peripheral blood Th1 (IFN-γ)/Th2 (IL-4) at 3

6

9

12 d were decreased obviously (P<0.01

P<0.05)

and dropped to the lowest point at 6 d

and then increased significantly at 12 d compared with that at 6 d (P<0.05). After treatment of QJM

cytokines IFN-γ at 3 d was slightly increased

without significance to the model

however those at 6

9

12 d were increased significantly (P<0.01

P<0.05) compared to the model. Cytokines level of TNF-α expression at 3

6

9

12 d were significantly decreased compared to those in the model group (P<0.01

P<0.05). Cytokine IL-3 at 3

6

9

12 d was slightly decreased

without significance to the model. Cytokines IL-4

IL-6

IL-10 at 3

6

9

12 d were significantly lower than those in the model group. Peripheral blood Th1 (IFN-γ)/Th2 (IL-4) at 3

6

9

12 d were significantly increased (P<0.01)

and that at 12 d was increased significantly compared with that at 3 d (P<0.01). Conclusion: QJM can control the infection of influenza virus through regulating Th type cytokine expression in infected mice

improving immune function and restoring the resistance to infection.