青蒿琥酯对肝纤维化大鼠肝脏基质金属蛋白酶-2表达的影响

来丽娜; 任泽恩; 宋晓亮; 刘芳; 郭春花; 范毅敏; 王黎敏

doi:10.11653/syfj2013110264

您当前的位置：

首页 >

文章列表页 >

青蒿琥酯对肝纤维化大鼠肝脏基质金属蛋白酶-2表达的影响

更新时间：2024-01-04

- 青蒿琥酯对肝纤维化大鼠肝脏基质金属蛋白酶-2表达的影响
- Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis
- 中国实验方剂学杂志 2013年19卷第11期页码：264-267
- 作者机构：
- 作者简介：
- 基金信息：
  
  山西省高校科技研究开发项目(20081032);长治市科技项目(20123057)
- DOI：10.11653/syfj2013110264
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
来丽娜, 任泽恩, 宋晓亮, 等. 青蒿琥酯对肝纤维化大鼠肝脏基质金属蛋白酶-2表达的影响[J]. 中国实验方剂学杂志, 2013,19(11):264-267.

LAI Li-na, REN Ze-en, SONG Xiao-liang, et al. Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 264-267.
来丽娜, 任泽恩, 宋晓亮, 等. 青蒿琥酯对肝纤维化大鼠肝脏基质金属蛋白酶-2表达的影响[J]. 中国实验方剂学杂志, 2013,19(11):264-267. DOI： 10.11653/syfj2013110264.

LAI Li-na, REN Ze-en, SONG Xiao-liang, et al. Effects of Artesunate on Matrix Metalloproteinase 2 Expression in Rat of Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(11): 264-267. DOI： 10.11653/syfj2013110264.

摘要

目的: 探讨青蒿琥酯(Art)影响基质金属蛋白酶-2(MMP-2)的抗肝纤维化作用机制。方法: 采用二甲基亚硝胺(DMN)ip 6周

制备大鼠肝纤维化模型。动物随机分为6组:正常对照组、模型对照组、Art(5

45 mg·kg-1)组、阳性对照秋水仙碱(Col

0.1 mg·kg-1)。检测肝组织中羟脯氨酸(Hyp)的含量、动物血清透明质酸(HA)

层连蛋白(LN)

Ⅲ型前胶原肽(PⅢP)水平及肝组织MMP-2 mRNA和蛋白的表达。结果: 与模型组相比

Art各组能降低肝组织Hyp含量(P<0.01)

同时血清HA

PⅢP

肝组织MMP-2-mRNA和蛋白的表达水平下降(P<0.05

P<0.01)。结论: Art能有效抑制DMN诱导的大鼠肝纤维化及肝脏中MMP-2的表达。

Abstract

Objective: To investigate the effects of artesunate(Art) on Matrix Metalloproteinase-2(MMP-2) activity

explore the mechanism against liver fibrosis. Method: Liver fibrosis model of rats was induced by injecting N-nitroso dimethylamine (DMN) for 6 weeks. Rats were randomly divided into 6 groups: the normal group

the model group

the positive control group treated by colchicine(0.1 mg·kg-1·d-1)

and the three Art groups(5

45 mg·kg-1·d-1). The content of hydroxyproline (Hyp) of liver tissue

hyaluronic acid (HA)

laminin (LN)

collagen type Ⅲ peptide (PⅢP) of serum were analyzed. Expressions of MMP-2 protein and mRNA were assayed by Western blot and RT-PCR. Result: The content of Hyp in liver and HA

PIIIP of serum in artesunate group were remarkably decreased and expressions of MMP-2 protein and mRNA were down-regulated compared with the model group. Conclusion: Artesunate can effectively inhibit the progress of hepatic fibrosis and the expression of MMP-2 in rats of liver fibrosis induced by DMN.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

铁皮石斛多糖基于Notch信号通路抗大鼠肝纤维化作用及其超声评价

调肝化纤丸对乙型肝炎肝纤维化的防治临床疗效及对弥散加权成像的影响

青蒿琥酯联合用药及作用机制研究进展

中医药基于NF-κB信号通路治疗肝纤维化的研究进展

基于自噬探讨中医药抗肝纤维化的研究进展