Objective: To optimize purification technology of total saponins from Allium sativum with macroporous resin. Method: With transfer rate of total saponins as index

single factor tests were adopted to optimize purification process parameters

such as type of macroporous resin

adsorption capacity of resin (adsorption capacity

the concentration of sample solution

pH and adsorption speed) and elution performance (the concentration

flow rate and volume of eluent). Result: Optimized purification technology was as following:selected AB-8 macroporous resin

ratio of crude drug-resin 1.3:1

the concentration of sample solution 0.8 g·mL-1

pH 7

adsorption speed 2 BV·h-1

removed impurities with 30% ethanol

eluted with 5 BV of 95% ethanol

elution speed 3 BV·h-1.Under these conditions

purity of total saponins was over 65%. Conclusion: AB-8 macroporous resin could be adopted to purify total saponins from A. sativum

this optimized purification technology was stable and feasible.