响应面法优化鹿衔草总黄酮的酶联半仿生法提取工艺

雒馨怡; 陈晓辉; 毕开顺; 李伟

doi:10.11653/syfj2013120019

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响应面法优化鹿衔草总黄酮的酶联半仿生法提取工艺

更新时间：2024-01-04

- 响应面法优化鹿衔草总黄酮的酶联半仿生法提取工艺
- Optimization of Enzyme-linked Semi-bionic Extraction Technology of Total Flavanoids from by Response Surface Methodology
- 中国实验方剂学杂志 2013年19卷第12期页码：19-22
- 作者机构：
- 作者简介：
- 基金信息：
  
  辽宁省教育厅科学技术研究项目(L2010535)
- DOI：10.11653/syfj2013120019
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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雒馨怡, 陈晓辉, 毕开顺, 等. 响应面法优化鹿衔草总黄酮的酶联半仿生法提取工艺[J]. 中国实验方剂学杂志, 2013,19(12):19-22.

LUO Xin-yi, CHEN Xiao-hui, BI Kai-shun, et al. Optimization of Enzyme-linked Semi-bionic Extraction Technology of Total Flavanoids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 19-22.
雒馨怡, 陈晓辉, 毕开顺, 等. 响应面法优化鹿衔草总黄酮的酶联半仿生法提取工艺[J]. 中国实验方剂学杂志, 2013,19(12):19-22. DOI： 10.11653/syfj2013120019.

LUO Xin-yi, CHEN Xiao-hui, BI Kai-shun, et al. Optimization of Enzyme-linked Semi-bionic Extraction Technology of Total Flavanoids from by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 19-22. DOI： 10.11653/syfj2013120019.

摘要

目的: 优选鹿衔草中总黄酮的酶联半仿生法提取工艺。方法: 以总黄酮提取率为指标

在单因素试验基础上

选取加酶量、料液比、酶解时间、提取时间、提取温度5个因素进行Box-Behnken中心组合设计

利用响应面法优选提取工艺。结果: 优选的提取工艺为加酶量1.25 mg·g-1

料液比1:65

酶解时间1.0 h

提取时间1.75 h

提取温度85℃

总黄酮提取率达5.54%

与预测值5.68%较为接近。结论: 采用响应面法优选的提取工艺合理可行

具有良好预测性。

Abstract

Objective: To optimize enzyme-linked semi-bionic extraction technology of total flavanoids from Pyrola decorata. Method: With yield of total flavanoids as index

based on single factor test

Box-Behnken central composite design was adopted by selecting the amount of enzyme

material-liquid ratio

enzymolysis time

extraction time and temperature as factors

extraction technology was optimized by response surface methodology. Result: Optimum extraction conditions were as follows:the amount of enzyme 1.25 mg·g-1

ratio of material to liquid 1:65

enzymolysis time 1.0 h

extraction time 1.75 h

extraction temperature 85℃. Under this conditions

yield of total flavanoids was up to 5.54%

which was close to the predicted value(5.68%). Conclusion: Optimized technology was reasonable and feasible with good predictability.

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