泥胡菜总黄酮大孔树脂纯化工艺优化

林珊; 刘仁根; 曾建伟; 邹秀红; 吴锦忠

doi:10.11653/syfj2013120034

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泥胡菜总黄酮大孔树脂纯化工艺优化

更新时间：2024-01-04

- 泥胡菜总黄酮大孔树脂纯化工艺优化
- Optimization of Purification Technology of Total Flavonoids from by Macroporous Resin
- 中国实验方剂学杂志 2013年19卷第12期页码：34-36
- 作者机构：
- 作者简介：
- 基金信息：
  
  福建省自然科学基金课题(2010J01198);福建省重点高校服务海西项目(No.5)
- DOI：10.11653/syfj2013120034
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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林珊, 刘仁根, 曾建伟, 等. 泥胡菜总黄酮大孔树脂纯化工艺优化[J]. 中国实验方剂学杂志, 2013,19(12):34-36.

LIN Shan, LIU Ren-gen, ZENG Jian-wei, et al. Optimization of Purification Technology of Total Flavonoids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 34-36.
林珊, 刘仁根, 曾建伟, 等. 泥胡菜总黄酮大孔树脂纯化工艺优化[J]. 中国实验方剂学杂志, 2013,19(12):34-36. DOI： 10.11653/syfj2013120034.

LIN Shan, LIU Ren-gen, ZENG Jian-wei, et al. Optimization of Purification Technology of Total Flavonoids from by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 34-36. DOI： 10.11653/syfj2013120034.

摘要

目的: 优选大孔树脂纯化泥胡菜总黄酮的工艺条件。方法: 采用静态吸附-洗脱试验筛选大孔树脂型号

动态吸附法优化大孔树脂纯化泥胡菜总黄酮工艺参数。结果: 优选的纯化工艺为选用D101型树脂

上样液质量浓度1.5 g·L-1

上样速率3 BV·h-1

上样体积4 BV

上样液pH 5

加50%乙醇5 BV以2 BV·h-1流速洗脱

泥胡菜总黄酮纯度达47.8%。结论: D101型大孔树脂对泥胡菜总黄酮具有良好的纯化效果。

Abstract

Objective: To optimize purification technology conditions of total flavonoids from Hemisteptia lyrata by macroporous resin. Method: Two models of macroporous resins were selected by static adsorption-elution test

purification technology of total flavonoids from H. lyrata with macroporous resin was optimized by dynamic adsorption method. Result: D101 resin was adopted

optimized purification technology was as following:the concentration of sample solution 1.5 g·L-1 with pH 5

adsorption velocity 3 BV·h-1

sample volume 4 BV

then eluted with 5 BV 50% ethanol at the speed of 2 BV·h-1

collected eluate.Under these conditions

yield of total flavonoids was 47.8%. Conclusion: D101 macroporous resin could purify total flavonoids from H. lyrata effectively.

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