蜘蛛香总缬草三酯提取物的纯化工艺优选

郜红利; 谭玉柱

doi:10.11653/syfj2013120037

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蜘蛛香总缬草三酯提取物的纯化工艺优选

更新时间：2024-01-04

- 蜘蛛香总缬草三酯提取物的纯化工艺优选
- Optimization of Purification Technology of Total Valepotriates from Extract of
- 中国实验方剂学杂志 2013年19卷第12期页码：37-39
- 作者机构：
- 作者简介：
- 基金信息：
  
  湖北省自然科学基金重点项目(2010CDA047)
- DOI：10.11653/syfj2013120037
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
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郜红利, 谭玉柱. 蜘蛛香总缬草三酯提取物的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(12):37-39.

GAO Hong-li, TAN Yu-zhu. Optimization of Purification Technology of Total Valepotriates from Extract of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 37-39.
郜红利, 谭玉柱. 蜘蛛香总缬草三酯提取物的纯化工艺优选[J]. 中国实验方剂学杂志, 2013,19(12):37-39. DOI： 10.11653/syfj2013120037.

GAO Hong-li, TAN Yu-zhu. Optimization of Purification Technology of Total Valepotriates from Extract of [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 37-39. DOI： 10.11653/syfj2013120037.

摘要

目的: 优选蜘蛛香总缬草三酯提取物的纯化工艺。方法: 采用动态吸附-洗脱试验筛选大孔树脂型号

通过单因素试验考察上样量、洗脱流速、洗脱溶剂及其用量对总缬草三酯富集纯化工艺的效果。结果: 采用ADS-8型大孔吸附树脂

优选的纯化工艺为上样量42.3 mg·g-1

上样液质量浓度1.692 g·L-1

用30%乙醇除杂

用95%乙醇6 BV洗脱

洗脱流速2 mL·min-1

总缬草三酯纯度51%

出膏率26%。结论: 优选的纯化工艺稳定可靠、重复性好。

Abstract

Objective: To optimize purification process of total valepotriates from extract of Valeriana jatamansi. Method: Models of macroporous resin was screened by dynamic adsorption-elution test

effect of sample volume

elution flow rate

elution solvent and its amount on purification process of total valepotriates was investigated by single factor test. Result: ADS-8 macroporous resin was adopted

optimized purification technology was as following:the concentration of sample solution 1.692 g·L-1

elution flow rate of 2 mL·min-1

sample capacity 42.3 mg·g-1

after eluted with 30% ethanol and 6 BV of 95% ethanol

yield of total valepotriates was 26% and purity was 51%. Conclusion: Optimized purification technology was stable

reliable and reproducible.

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