Objective: To investigate in vitro transdermal behavior of hydroxysafflor yellow A(HSYA) in Guyu prescription. Method: Percutaneous absorption test was performed on excised mice skin in Franz diffusion cell

with the content of HSYA as index

effect of the concentration of ethanol and drug on transdermal property of HSYA was investigated. Result: The accumulation skin permeation ratio of HSYA with 20% ethanol

50% ethanol and 70% ethanol were 1.26%

1.69%

5.28%

respectively;And results of 0.5

1

2 g·mL-1 of drug solution were 1.06%

1.26%

1.50%

respectively.Increasing of the concentration of ethanol and drug solution were conducive HSYA through skin;the concentration of 1 g·mL-1 and 70% were selected. Conclusion: 70% ethanol could significantly promote transdermal property of HSYA

it could provide a reference for preparing Guyu liniments.