墨旱莲多糖的酶法提取工艺优选

杨春静; 张杰; 张华锋; 刘炯; 杨云; 杨璐

doi:10.11653/syfj2013120063

您当前的位置：

首页 >

文章列表页 >

墨旱莲多糖的酶法提取工艺优选

更新时间：2024-01-04

- 墨旱莲多糖的酶法提取工艺优选
- Optimization of Enzymatic Extraction Technology of Polysaccharides from
- 中国实验方剂学杂志 2013年19卷第12期页码：63-65
- 作者机构：
- 作者简介：
- 基金信息：
  
  2010河南省郑州市科技攻关项目(10PTGS486-1)
- DOI：10.11653/syfj2013120063
  中图分类号：
- 纸质出版日期：2013
- 稿件说明：
移动端阅览
杨春静, 张杰, 张华锋, 等. 墨旱莲多糖的酶法提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(12):63-65.

YANG Chun-jing, Zhang Jie, ZHANG Hua-feng, et al. Optimization of Enzymatic Extraction Technology of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 63-65.
杨春静, 张杰, 张华锋, 等. 墨旱莲多糖的酶法提取工艺优选[J]. 中国实验方剂学杂志, 2013,19(12):63-65. DOI： 10.11653/syfj2013120063.

YANG Chun-jing, Zhang Jie, ZHANG Hua-feng, et al. Optimization of Enzymatic Extraction Technology of Polysaccharides from [J]. Chinese journal of experimental traditional medical formulae, 2013, 19(12): 63-65. DOI： 10.11653/syfj2013120063.

摘要

目的: 优选酶法提取墨旱莲多糖的工艺条件。方法: 采用苯酚-硫酸法测定多糖含量

以多糖提取率和蛋白质含量的综合评分为指标

采用单因素试验筛选酶的种类。以多糖总提取率为指标

在单因素试验基础上

采用正交试验考察酶用量、酶解温度、酶解时间、pH对墨旱莲多糖提取工艺的影响。结果: 选用纤维素酶

最佳提取工艺条件为pH 5.0

纤维素酶用量3%

酶解时间3 h

酶解温度50℃。结论: 酶解水提法可显著提高墨旱莲多糖的提取率。

Abstract

Objective: To optimize enzymatic extraction technology conditions of polysaccharides from Eclipta prostrasta. Method: Composite score of yield of polysaccharides and the content of protein as index

type of enzyme was screened by single factor test.With total yield of polysaccharides as index

based on single factor tests

effect of the amount of enzyme

hydrolysis time and temperature on extraction technology of polysaccharides was investigated by orthogonal test. Result: Cellulose enzyme was adopted

optimum extraction technology was as following:pH 5.0

the amount of cellulose enzyme 3%

hydrolysis time 3 hours and temperature at 50℃. Conclusion: Yield of polysaccharides from E. prostrasta could significantly increase by enzymolysis water extraction method.

关键词

Keywords

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

正交试验法优选补肾益髓颗粒提取工艺

正交试验法优选复方茜草胶囊提取工艺的研究

三妙消痛贴的提取工艺研究

多指标正交试验优选升麻提取工艺